Lauryl Sulphate Tryptose Broth (500 gram)
LV002-02049
Productomschrijving
Oxoid Lauryl Sulphate Tryptose Broth.
Cronobacter (formerly Enterobacter sakazakii) are Gram-negative rod-shaped members of the Family Enterobacteriaceae that have been implicated in outbreaks of disease in premature infants (neonates), causing sepsis, meningitis and necrotising enterocolitis.
Cronobacter spp. have been isolated at low levels from powdered infant formulae, and the organisms’ high tolerance to desiccation provides a competitive advantage in the dry environments of milk powder factories, increasing the risk of product contamination.
In response to concerns over this pathogen, a collaborative technical specification for the detection of Cronobacter in milk and milk products was jointly issued by ISO and the IDF. as ISO/TS22964:2006. This recommended pre-enrichment in BPW, followed by selective enrichment in mLST/vancomycin (mLSTv) broth and plating on Enterobacter sakazakii Isolation Agar (CM1134). Positive colonies are confirmed on the basis of sugar utilisation, decarboxylase activity, as well as yellow pigmentation.
MLST broth was originally described by Guillaume-Gentil et al. for the selective enrichment of Cronobacter. The high salt concentration of the medium (34g/l) restricts the growth of other members of the Enterobacteriaceae, and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments. A careful blend of peptones and lactose ensures Cronobacter remain viable in the presence of these selective agents
Specificaties
| Inhoudsgegevens | |
|---|---|
| Inhoud in gram | 500 |
| Productspecifiek | |
|---|---|
| Mediatype | Lauryl Sulphate Tryptose Broth |
| Vorm | Poeder |